Scotland’s 1904 antarctic expedition made a unique contribution to science:

A number of emperor penguins, which were here very numerous, were captured. … To test the effect of music on them, Piper Kerr played to one on his pipes, — we had no Orpheus to warble sweetly on a lute, — but neither rousing marches, lively reels, nor melancholy laments seemed to have any effect on these lethargic phlegmatic birds; there was no excitement, no sign of appreciation or disapproval, only sleepy indifference.

— Rudmose Brown et al., The Voyage of the “Scotia,” 1906

(This has produced a memorable Wikipedia image caption.)